Anna Pittermannová – Diploma thesis summary

Investigation of selective particle adhesion to biological subtrates

Nowadays the development of composite nano- and micro particles is an active area of research. This interest is growing especially for their potential use in targeted delivery of active substances. System of targeted delivery particles is very useful especially in pharmaceutics industry. This work is focused on surface modification of silica nanoparticles with specific antibody IgG-M75 and their specific bioadhesion. Adhesion properties were studied on substrates modified by domain PG-MBP of antigen CA IX, or on cancer cells which expressed this antigen. Adhesion properties were studied in different flow rates. Adhesion of nanoparticles modified by specific antibody was compare with adhesion of nonspecific nanoparticles. It was found that the specific modification of nanoparticles with antibody-like protein is a feasible tool for targeting of tumor cells specifically.